The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing Screening techniques for plant nutrient efficiency: Philosophy and methods. In: El Bassam, N., Dambroth, M., Loughman, B.C. (eds) Genetic Aspects of Plant Mineral Nutrition. Screening techniques for plant nutrient efficiency: Philosophy
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Establishment of screening system for P efficiency of soybeans at the seedling stage. As we all know, the yield screening method under low-phosphorus An ecient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cellsAn efficient direct screening system for microorganisms
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Based on established methods in animal cell cultures, we also envision that CRISPR screens could be combined with machine learning to design gRNAs with higher Screening Efficiency. Improvements in dry screening efficiency and processing will be extremely helpful in arid areas. From: The Coal Handbook (Second Screening Efficiency an overview ScienceDirect Topics
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To monitor photosynthetic performance in algae and higher plants, several photosynthetic parameters, such as photochemical efficiency of PSII,electron transport,The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at Discriminated sgRNAs-Based SurroGate System
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For the first time in plant micropropagation, we have developed a miniaturized and automated screening system to meet high-throughput standards.Screening Plant. Methods for screening plant extracts for the presence of α-glucosidase inhibitors have been optimized, e.g., HPLC microfractionation—where HPLC fractions Screening Plant an overview ScienceDirect Topics
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The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high efficiency. Despite the efforts have made on Artemisia annua transient expression, achieving high-throughput gene functional Background Photosynthesis is one of the most important biological reactions and forms the basis of crop productivity and yield on which a growing global population relies. However, to develop improved plant cultivars that are capable of increased productivity, methods that can accurately and quickly quantify photosynthetic efficiency in Chlorophyll fluorescence imaging captures photochemical efficiency
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A group of hybrid nozzles is selectively mounted at different locations of the crushing and screening plant for achieving better efficiency in suppressing dust. The spraying operation of hybrid nozzles is controlled by flow and pressure control units (FPUs) having complete electronic control circuitry to facilitate spraying operation of hybridWith higher editing efficiencies, screening of individual plants for the desired edits by PCR is a viable option. When creating specific edits, this can be done via an edit-specific PCR assay. Ma X, Zhang X, Liu H, Li Z (2020) Highly efficient DNA-free plant genome editing using virally delivered CRISPR-Cas9. Nat Plant 6:773–779. ArticleDetection of genome edits in plants—from editing to seed
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Provide you with information to promote a safer, more cost efficient operation. • Topics covered to include: • Performance and carrying capacity. • 95% objective screening efficiency, with no specific or defined reference to the allowable percentage of oversize or undersize in the product.The third level allows an efficient screening of PSB that could efficiently solubilize P along with increasing plant growth. The improvement of plant growth could be associated with multiple plant growth-promoting traits that PSB could exhibit rather than PSB solubilization traits [126], [127].Phosphate bacterial solubilization: A key rhizosphere driving
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ecological background of searching for more nutrient efficient plants. Breeding for nutritional efficiency, however, is still hampered by the lack of reliable screening criteria. Therefore, there is a necessity to define properties and metabolic activities of significance to nutrient efficiency. The following aspects will beWith the advent of multiple omics and Genome-Wide Association Studies (GWAS) technology, genome-scale functional analysis of candidate genes is to be conducted in diverse plant species. Construction of plant binary expression vectors is the prerequisite for gene function analysis. Therefore, it is of significance to develop a set of Development of a set of novel binary expression vectors for plant
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Development of high-throughput phenotyping technologies has progressed considerably in the last 10 years. These technologies provide precise measurements of desired traits among thousands of field-grown plants under diversified environments; this is a critical step towards selection of better performing lines as to yield, disease resistance, Electrostatic forces can also hinder screening efficiency in way of water attraction causing sticking or . one of such objects in minerals processing plants is the vibrating screen, Fig. 1.(PDF) ORE SEPARATION BY SCREENING ResearchGate
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The system has been implemented in an iron ore crushing and screening plant in India, and its efficacy has been evaluated for controlling dust emission. The installed dry fog system reduced dust concentration to 0.10–0.17 mg m−3 from the prevailing dust concentration of 0.62–1.73 mg m−3 in work zone areas, which was much below theRequest PDF Screening of potential phosphate solubilizing bacteria inoculants should consider the contrast in P bio-solubilization rate along with plant growth promotion and P use efficiencyScreening of potential phosphate solubilizing bacteria
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Transient expression systems in mesophyll protoplasts have been utilised in many plant species as an indispensable tool for gene function analysis and efficacious genome editing constructs. However, such a system has not been developed in Cannabis due to the recalcitrant nature of the plant to tissue culture as well as its illegal status for Soil salinity is a worldwide issue that affects wheat production. A comprehensive understanding of salt-tolerance mechanisms and the selection of reliable screening indices are crucial for breeding salt-tolerant wheat cultivars. In this study, 30 wheat genotypes (obtained from a rapid selection of 96 original varieties) were chosen to Evaluating and Screening of Agro-Physiological Indices for Salinity
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Conclusions. The development of genome editing technologies in plants enables a breadth of opportunities for plant breeding. Efficient, precise, and targeted mutagenesis via genome editing has laid the foundation for many next-generation breeding strategies that will revolutionize the future of agriculture.Phosphorus (P) is often an important limiting factor for crop yields, but rock phosphate as fertilizer is a non-renewable resource and expected to become scarce in the future. High P input levels in agriculture have led to environmental problems. One of the ways to tackle these issues simultaneously is improving phosphorus use efficiency (PUE) Improving phosphorus use efficiency in agriculture:
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The success of in vitro plant regeneration and the competence of genetic transformation greatly depends on the genotype of the species of interest. In previous work, we developed a method for theScreening Efficiency of Plant Base-Edited Cells. Mol. Plant. 13, 169–180. INTRODUCTION Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have been used with great success to modify the genomes of a wide variety of organisms (Jinek et al., 2012; Cong et al., 2013; Mali et Discriminated sgRNAs-Based SurroGate System Greatly
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Plant materials. Seeds of Arabidopsis thaliana (ecotype Columbia-0) were grown on half-strength Murashige and Skoog media (MS) [] supplemented with 0.6% (w/v) agarose for 7 d in controlled environmental conditions of 21 °C and a 16-h-light/8-h-dark photoperiod, with the light intensity of 150 μmol m −2 s −1.The seedlings were Background Astragalus mongolicus Bunge is used in traditional Chinese medicine and is thus cultivated in bulk. The cultivation of A. mongolicus requires a large amount of nitrogen fertilizer, increasing the planting cost of medicinal materials and polluting the environment. Isolation and screening of plant growth-promoting rhizobacteria (PGPR) Screening of high-efficiency nitrogen-fixing bacteria from
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